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Enzymes in RNA Science and Biotechnology Part B
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ISBN: 9780443239762
Published: 2023
Publisher: Academic Press
Language: English
Format: Hardback
Format: 9×6
Published: 2023
Publisher: Academic Press
Language: English
Format: Hardback
Format: 9×6
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<i>Enzymes in RNA Science and Biotechnology, Part B, Volume 692</i> in the <i>Methods in Enzymology</i> series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNA, Quantitative base-resolution DAMM-seq for mapping RNA methylations in tRNA and mitochondrial dsRNA, Discovering RNA modification enzymes using a comparative genomics approach, Functional analysis of tRNA modification enzymes using mutational profiling, and Fluorescent labeling of tRNA for rapid kinetic interaction studies with tRNA-binding proteins.<br><br>Other chapters cover Enzymatic Synthesis of RNA Standards for Mapping and Quantifying RNA Modifications in Sequencing Analysis, Characterizing RNase L-mediated mRNA decay in single cells, Characterization of RNase J, Pri-miRNA cleavage assays for the Microprocessor complex, The pre-miRNA cleavage assays for DICE, Methods for study of ribonuclease targeting chimeras, and Profiling the in vivo RNA interactome associated with the endoribonuclease RNase III in Staphylococcus aureus.
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